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Biblioteca(s): 	INIA Las Brujas.	
Fecha corriente: 	23/08/2018	
Fecha de la última actualización: 	23/08/2018	
Autoria: 	ABREO, E.; VALLE, D.; MUJICA, V.; ALTIER, N.	
Afiliación: 	EDUARDO RAUL ABREO GIMENEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DIANA VALLE LOPEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA VALENTINA MUJICA TELIZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NORA ADRIANA ALTIER MANZINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.	
Título: 	Pathogenicity and virulence factors of Lysinibacillus xylanilyticus and Bacillus spp. towards Argyrotaenia sphaleropa larvae (Lepidoptera).	
Complemento del título: 	Original contribution.	
Año de publicación: 	2018	
Fuente/Imprenta: 	Journal of Applied Entomology, 2018, p.1-11.	
DOI: 	10.1111/jen.12539	
Idioma: 	Inglés	
Notas: 	Article history: Received: 28 January 2018 / Accepted: 3 June 2018 / First published: 04 July 2018.	
Contenido: 	Abstract
Argyrotaenia sphaleropa is a lepidopteran pest in deciduous fruit orchards in Uruguay. The primary control method of these pests is the use of chemical insecticides; however, alternative strategies, friendlier to the environment and compatible with a regional programme of integrated pest management, are definitely needed. Biological control with the use of local entomopathogenic microorganisms could be a sustainable solution. In this study, a biological screening was conducted to characterize spore&#8208;forming bacilli for their capacity to kill larvae of this species. Bacillus thuringiensis, B. pumilus, B. aryabhattai, B. amyloliquefaciens and Lysinibacillus xylanilyticus were identified based on sequence analyses of 16S and partial GyrB gene. Lysinibacilus xylanilyticus showed the highest mortality towards first&#8208;instar larvae fed with diet treated with spore suspensions. Specific virulence factors like proteinaceous parasporal inclusions were observed not only in isolates of B. thuringiensis but also in L. xylanilyticus. Ordering of isolates based on multivariate analysis of putative secondary virulence factors like biosurfactant and biofilm production, haemolytic activity and motility, separated the virulent isolate of L. xylanilyticus. This is the first report of L. xylanilyticus causing mortality in A. sphaleropa or any other member of Lepidoptera. A search for more virulent strains of this species is envisaged.
@© 2018 Blackwell Verlag GmbH	
Palabras-Clave: 	16S; BIOFILM; CRY PROTEINS; HAEMOLYSIS; INTEGRATES PEST MANAGEMENT.	
Thesagro: 	TORTRICIDAE.	
Categoría del tema: 	--	
Registro original: 	INIA Las Brujas (LB)	
Ejemplares: 	LB - (101667-1) - PXI - AP - DD - PP/JR.APPLIED ENTOMOLOGY/2018 -  -  - DIS